Effect of encapsulated nitrate and elemental sulphur on methane production and microbial protein synthesis in beef cattle

pan SRUC seminar with Irene Cabeza, SRUC on 16th of March 2017.
Methane produced from domestic ruminant enteric fermentation is the largest anthropogenic source of CH4. Alternative sinks need to be investigated to redirect metabolic rumen hydrogen (H2) away from methanogenesis and provide an energetic gain for the host animal. This PhD project has focused on potential additives to reduce methane emissions in beef cattle
The main study presented investigated the effect of using EN as a replacement for urea (U) or dietary protein, plus the addition of inorganic S, on enteric CH4 emission, nutrient digestibility, N utilization and microbial protein synthesis from crossbred beef steers. In addition, nitrate toxicity and eating behaviour were investigated. This experiment was conducted at UNESP Brazil in 2015.Five crossbred Angus x Nellore steers fitted with permanent rumen cannulae were used in a 5×5 Latin square design with 21 day periods (14 days adaptation and 7 days measurement) Enteric CH4 production was measured from day 15 to 20 using the sulphur hexafluoride technique
The final experiment of my PhD project investigated the effects of nitrate addition to steers diets on methane production and rumen microbial community. The MiSeq Illumina platform have been used for community amplicon sequencing. The purpose is to identify changes in microbial populations related to the basal diet and nitrate addition, and to find correlations with methane production from these animals.

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